Helicon focus: fascinating 3D images in microscopy and macroscopyĬontrolling Sony Alpha cameras remotely in Live View mode from a tablet or smartphone: Sony’s PlayMemories Mobile app The Panasonic Lumix DC-G9 80 MP pro-grade camera and its performance in microscopy Testing report: Nikon Camera Control Pro2ĢD measuring with Canon EOS cameras (DSLR and DSLM) in microscopy:Ĭontrolling Olympus digital SLR cameras from your PC with the OLYMPUS Studio 2 software To sum up, we can recommend ImageJ as a highly useful software for our area of application that has the added bonus of being available for free.Ĭamera recommendation for microscopy application Depending on the requirements in each case, it can be used as a replacement or complement to other commercial software products. ImageJ works well with other programs, such as Canon Utility, Nikon Remote Control 2 and Capture One. A very interesting feature for us – image acquisition – is also supported under Acquisition of Cameras/Twain. Filters: Real Convolver, FFT, De-interlace, Auto Gammaĥ) The following tools are located in the Analyze menu:Ħ.) To measure a cell, for example, you need to choose the image measurement tool in the Plugins menu:Īdditional tools include: Brush Tool, Lasso Selection Tool, Pixel Inspection Tool, etc. The working language is English.ĭue to the modular nature of the program, additional functions can be implemented via plugins, which can be downloaded and installed from the website: ġ) As usual, open the image you want to work on with File/Openand import it into ImageJ:Ģ) Use the options available under Edit to perform a variety of standard tasks, such as Cut, Copy, Paste, Clear, Fill, Draw, Invert, etc.ģ) The Image menu contains a selection of image processing routines, such as:Ĥ) The following functions are included in the Process menu: ![]() When you first start it, you get only a small toolbar with a list of menu items that give access to most of the functions within the program. Installing ImageJ is easy: Download the ZIP file from and unpack the contents. Basic features include a wide range of measurement tools such as length measurement, angle measurement, surface calculation and much more. The program offers a huge array of functions and can be extended using plugins. It was created by US software developer Wayne Rasband, who continues to add features to the program. ImageJ is available as open-source software that is highly extensible and can be used free of charge. It is written in Java, which allows it to run on different operating systems like Windows, Mac and Linux. ImageJ is a powerful and versatile software package designed for scientific image processing and analysis. The source code for ImageJ is freely available.The Java ImageJ image editing and processing software in microscopy and macroscopy – a brief review Downloadable distributions are available for Microsoft Windows, Mac OS, Mac OS X, Linux, and the Sharp Zaurus PDA. ImageJ can be run as an online applet, a downloadable application, or on any computer with a Java 5 or later virtual machine. ![]() ImageJ's plugin architecture and built in development environment has made it a popular platform for teaching image processing. User-written plugins make it possible to solve many image processing and analysis problems, from three-dimensional live-cell imaging, to radiological image processing, multiple imaging system data comparisons to automated hematology systems. Custom acquisition, analysis and processing plugins can be developed using ImageJ's built-in editor and a Java compiler. ImageJ was designed with an open architecture that provides extensibility via Java plugins and recordable macros. ImageJ is a public domain, Java-based image processing program developed at the National Institutes of Health.
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